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VITROCELL ®  Cloud Alpha

For 6, 12, 24-well inserts 

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Cloud Alpha

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VITROCELL ®  Cloud Alpha 

VITROCELL ® 노출 시스템은 가스, 복잡한 혼합물, 나노입자 및 섬유와 같은 공기 중 물질에 직접 노출되는 박테리아에 대한 연구를 용이하게 하도록 특별히 설계 및 제작되었습니다. 이 시스템은 인간의 생리적 노출 조건을 확실하게 시뮬레이션합니다

VITROCELL® Cloud Alpha MOVE

VITROCELL ® Cloud Alpha MOVE는 우리의 혁신이며 세포 배양의 완전 자동 노출에서 또 다른 큰 도약을 제시합니다. 하나의 실험에서 용량-반응 관계를 얻기 위한 이상적인 장치입니다. 이 개발은 잘 알려져 있고 자주 게시되는 VITROCELL ® 클라우드 형식을 기반으로 합니다. 이 기능은 올인원 제어 장치로 완전히 자동화된 프로세스를 가능하게 합니다.

​

자동화에 중점을 두고 개발

 

Cloud Alpha MOVE는 Corning ® HTS Transwell ® 96-웰 투과 지원 시스템과 12- 및 24-웰 크기의 삽입물에서 포유류 세포 배양을 노출할 수 있습니다. 클라우드 시스템은 용액 및 현탁액의 분무화에 적합합니다. 응용 분야는 흡입 약물의 스크리닝, 화학 물질 또는 나노 입자와 같은 흡입 물질의 독성 테스트 및 바이러스 연구입니다.

 

세 가지 유형의 분무기 선택

 

액적 MMAD 범위가 2.5–6.0 µm, 2.5–4.0 µm, 4.0–6.0 µm인 3가지 유형의 진동 메쉬 Nebulizer와 9.0–12.0 µm의 특수 버전이 함께 제공됩니다. 이 장치는 소량의 테스트 재료를 사용할 수 있을 때마다 테스트에 특히 적합합니다.

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Exposure at the Air/Liquid Interface

Air-Liquid-Cloud-Alpha-1-960x640.png

Air/Liquid Cultivation
and Exposure in Exposure Module

A: Direct and controlled ­exposure of test ­atmosphere to cells
B: Cells on membrane
C: Media below cells

In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired inter­action of the formerly airborne substances with the culture media.

Cell systems cultivated on membrane inserts are exposed at the Air/Liquid Interface (ALI) so that the test substances are received directly. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

96-well: 12 Individual Doses

Sequential Exposure of Corning® HTS Transwell® 96-Well Permeable Support Systems in up to
12 steps @8 replicates.

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24-well: 6 Individual Doses

Sequential Exposure of 24-well sized culture inserts in up to 6 steps @4 replicates.

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12-well: 6 Individual Doses

Sequential Exposure of 12-well sized culture inserts in up to 4 steps@3 replicates.

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For each of the exposures applies: After each nebulization the exposure chamber is automatically evacuated by vacuum pump to enable equal exposure conditions for the following nebulisation.

More about VITROCELL Cloud Alph Move

Controlled inlet flow
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VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

Dosimetry using Quartz Crystal Microbalance (QCM)
QCM-CLOUD-ALPHA-MOVE-960x640.jpg.jpeg

The Quartz Crystal Microbalance sensor is integrated in the Cloud Alpha MOVE exposure module. It is capable of measuring the deposited mass in nanogram/cm2. Results are recorded in the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.

Features

  • Fully automated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts

  • Aerosolisation defined by nebulization time or volume

  • Integrated controller for aerosol generator

  • Optional integrated microbalance controller

  • Programmable experiment recipes

  • Output rate database for nebulizers

  • Heating system

  • PowerVent function: evacuation of residual aerosols via vacuum pump

  • Designed for screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research

VITROCELL Cloud Alpha PowerVent

일반적인 클라우드 노출은 3회 연속 분무가 가능합니다. PowerVent 옵션을 사용하면 추가 분무를 추가하여 용량을 늘릴 수 있습니다. 각 분무 후 습기는 새 분무를 시작하기 전에 짧은 환기 기간으로 제거됩니다. 배기 기능은 소프트웨어에서 편집할 수 있습니다. PowerVent 장치는 통합 진공 펌프와 함께 바로 사용할 수 있는 상태로 배송됩니다.

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PowerVent System Interface

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Phase 1: Emission Of Cloud
Phase 2: Homogeneous Mixing
Phase 3: Gravitational Settling
Phase 4: Venting of Cloud
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Phase 5: Additional Emission
Phase 6: Homogeneous Mixing
Phase 7: Gravitational Settling
Phase 8: Venting of Cloud

PowerVent Components

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HEPA filter for filtered air

PowerVent adapter

HEPA filter

PowerVent Unitwith vacuum pump

Features

  • Available for all Cloud Alpha systems

  • Fast removal of residual ­gaseous compounds

  • Allows > 3 consecutive ­nebulizations

  • Integrated vacuum pump

  • Function can be edited in the softwar

VITROCELL® Cloud Alpha Max

VITROCELL ® Cloud Alpha MAX는 클라우드 제품군의 최신 혁신 제품으로 세포 배양의 자동화된 노출에서 큰 도약을 보여줍니다. 높은 처리량 테스트와 사용 편의성을 결합합니다. 이 개발은 잘 알려져 있고 자주 게시되는 VITROCELL ®  클라우드 형식(6, 12 및 24웰)을 기반으로 합니다. 이 기능은 올인원 제어 장치로 완전히 자동화된 프로세스를 가능하게 합니다. Air/Liquid Interface 에서의 일상적인 실험이 그 어느 때보다 쉬워졌습니다.

새로운 Cloud Alpha MAX 시리즈는 수많은 고객 요청의 결과로 개발되었으며 이제 6-, 12- 또는 24-well 크기의 세포 배양 삽입물에서 포유류 세포 배양을 노출할 수 있습니다. 모든 상용 브랜드가 지원됩니다.

클라우드 시스템은 용액 및 현탁액의 분무화에 적합합니다. 가능한 응용 분야는 흡입 약물의 스크리닝, 화학 물질 또는 나노 입자와 같은 흡입 물질의 독성 테스트 및 바이러스 연구입니다.

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Exposure at the Air/Liquid Interface

Air-Liquid-Cloud-Alpha-1-960x640.png

Air/Liquid Cultivation
and Exposure in Exposure Module

A: Direct and controlled ­exposure of test ­atmosphere to cells
B: Cells on membrane
C: Media below cells

In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired inter­action of the formerly airborne substances with the culture media.

Cell systems cultivated on membrane inserts are exposed at the Air/Liquid Interface (ALI) so that the test substances are received directly. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

Ideal for small quantities of test substance

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The VITROCELL® Cloud Alpha MAX is designed for lower nebulization volumes and very high deposition efficiency. This is important when only small quantities of material are available or when expensive test substances need to be tested. The non-cell exposed area is reduced significantly by direct nebulization into exposure chambers with reduced chamber volume. A set of 3 nebulizers allows for simultaneous testing of 3 concentrations, or a combination of Microbalance, control liquid and suspension of the test substance.

How the Vitrocell  Cloud works

After pipetting the suspension, exposure to the cell cultures takes place in three phases:

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Phase 1: Emission Of Cloud

Phase 2: Homogeneous Mixing

Phase 3: Gravitational Settling

More about VITROCELL Cloud Alpha MAX

Choice of three types of nebulizers
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The system comes with a choice of 3 types of vibrating mesh nebulizers having droplet MMAD ranges of 2.5 – 6.0 µm, 2.5 – 4.0 µm and 4.0 – 6.0 µm. Recommended nebulisation volumes are 10 – 40 µl. So the device is particularly suitable for testing whenever small quantities of testing materials are available.

Dosimetry using Quartz Crystal Microbalance (QCM)
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The QCM sensor is integrated in the Cloud Alpha MAX exposure module. It is capable of measuring the deposited mass at a resolution of 10 nanogram/cm2 per second. Results are reported online by the VITROCELL Monitor Software. Data is presented in graphs and stored in MS Excel®.

Insert-Sets
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All commercial brands are supported.

Features

  • ≤ 40 µl per exposure and well

  • High deposition efficiency

  • Cloud dynamics by vibrating mesh nebulizers

  • Easy handling with no external air-flow required

  • High reproducibility

  • Air Liquid Interface (ALI) exposure

  • Direct and efficient guidance of substance exposure to the cells

VITROCELL® Cloud Alpha TRIO

VITROCELL ® Cloud Alpha TRIO는 세포 배양 노출에 있어 또 다른 혁신적인 도약을 제시합니다. 이 다용도 장치를 사용하면 단일 실험에서 세 가지 용량 관계를 얻을 수 있거나 세 가지 다른 물질을 동시에 사용하는 작업이 용이해집니다. 후자의 절차는 더 많은 수의 다양한 물질을 테스트하고 비교해야 하는 사용자에게 특히 흥미로울 수 있습니다. 개발은 잘 알려지고 자주 게시되는 VITROCELL ® Cloud 형식을 기반으로 합니다.

​다양성에 중점을 두고 개발되었습니다. 

새로운 Cloud Alpha TRIO는 12웰 및 24웰 크기의 삽입물에 포유류 세포 배양물을 노출시킬 수 있습니다. 클라우드 시스템은 용액 및 현탁액의 분무에 적합합니다. 응용 분야는 흡입성 약물의 스크리닝, 화학물질이나 나노입자와 같은 흡입 물질의 독성 평가, 바이러스 연구 수행입니다.

​세 가지 유형의 분무기 중 선택
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2.5~6.0μm, 2.5~4.0μm, 4.0~6.0μm의 액적 MMAD 범위와 9.0~12.0μm의 고급 버전을 갖춘 세 가지 유형의 진동 메시 분무기를 선택할 수 있습니다. 이 장치는 제한된 양의 테스트 재료를 사용할 수 있을 때마다 테스트하는 데 특히 적합합니다

Exposure at the Air/Liquid Interface

Air-Liquid-Cloud-Alpha-1-960x640.png

Air/Liquid Cultivation
and Exposure in Exposure Module

A: Direct and controlled ­exposure of test ­atmosphere to cells
B: Cells on membrane
C: Media below cells

In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired inter­action of the formerly airborne substances with the culture media.

Cell systems cultivated on membrane inserts are exposed at the Air/Liquid Interface (ALI) so that the test substances are received directly. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

More about VITROCELL Cloud Alpha TRIO

12-well: Three individual doses or three different substances
12-well-cloud-alpha-trio-960x640.webp

Exposure of 12-well sized culture inserts in three rows at four replicates.

24-well: Three individual doses or three different substances
24-well-cloud-alpha-trio-960x640.webp

Exposure of 24-well sized culture inserts in three rows at four replicates.

Dosimetry using Quartz Crystal Microbalance (QCM)
QCM-CLOUD-ALPHA-MOVE-960x640.jpg.jpeg

The Quartz Crystal Microbalance sensor is integrated in the Cloud Alpha MOVE exposure module. It is capable of measuring the deposited mass in nanogram/cm2. Results are recorded in the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.

Insert-Sets
selection-of-inserts-12well_24well-480x320.jpg.jpeg

All commercial brands are supported.

Features

  • Suitable for nebulization of solutions and suspensions

  • Exposure of 12- and 24- well sized cell culture inserts

  • Exposure of three different dose ranges in one experiment or of three different substances in one experiment

  • Optionally, up to three sQCM 12 microbalances can be connected to one controller

  • Heating system

  • Optional PowerVent function: evacuation of residual aerosols via vacuum pump

  • Designed for screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research

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