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Highest Throughput
The VITROCELL® Highest Throughput Exposure Systems are designed to maximize efficiency and capacity in inhalation toxicology studies. They support a large number of samples simultaneously, enabling extensive and detailed analysis. These systems are particularly attractive to customers because they provide high-volume data collection, reducing time and costs associated with large-scale toxicological assessments while maintaining accuracy and reliability.
VITROCELL® 24/48
Exposure System
Exposure System for 24- well sized inserts allowing up to seven doses plus sham control simultaneously.
VITROCELL® 48/48
Plus Exposure System
Exposure System for 24-,12-,6- well sized inserts and 35mm Petri dishes allowing up to seven doses plus sham control simultaneously.
VITROCELL® 96
. The system authentically simulates the conditions of human physiological exposure. Up to 11 dilutions with 8 replicates are used for for exposure to test substances and the 12th insert row for clean air control with 8 replicates.
VITROCELL® 24/48 Exposure System
for 48 cell culture inserts (24-well size)
VITROCELL® 24/48
Stainless Steel Exposure Module
The VITROCELL® 24/48 module system has been specifically designed and engineered to facilitate the research of human cell cultures in direct exposure to airborne substances such as gases, complex mixtures, nanoparticles and fibers. The system authentically simulates the conditions of human physiological exposure.
7 dilutions with 6 inserts each are used for for exposure to the substances and 6 inserts in the same system for clean air control. Each dilution represents a different dose concentration, so that a complete dose / response profile can be obtained in one experiment.
VITROCELL® 24/48
Climatic Chamber
The Climatic Chamber enables a condensation-free operation. It accomodates all required components of the VITROCELL® 24/48 System. The humidification station, pre-heating system for dilution air and flow controllers are mounted inside the chamber for constant temperature and increased humidity output.
VITROCELL® 24/48
8 rows @ 6 replicates
The base module with 48 wells (24-well-size) is made of electropolished stainless steel. An optional transparent control window facilitates the external monitoring of media levels and may be easily removed from the integrated heating base. Temperature can be programmed individually.
VITROCELL® 24/48
48 inlets with indidual vacuum flow
Special sealing and connection mechanisms guarantee a hermetic connection of the base module with the aerosol exposure top.
The aerosol exposure top guides the test atmosphere at an individually controlled flow rate to each cell culture membrane.
VITROCELL® 24/48 Controlled inlet flow
VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
VITROCELL® 24/48
Integrated Humidification Station
The module has been designed for 24-well sized cell culture inserts.
VITROCELL® 24/48 Features
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Base module for 24-well sized inserts
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8-fold Dilution/Distribution System
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8 mass flow controllers for humidified air
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Flow controllers mounted inside of chamber
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7 dilutions @ 6 replicates
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1 clean air control @ 6 replicates
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Electronic heating system for base module
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Climatic Chamber for condensation-free operation if using liquid aerosols
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Housing with double-doors
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Heating systems with two RH/T sensors for temperature monitoring
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Integrated humidification system
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Optional dosimetry compartments
VITROCELL® 24/48
Integrated Dilution Systems
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New support and quick-mounting mechanism of dilution systems and exposure top for easier handling.
VITROCELL® 24/48
Integrated Dilution Systems
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The Humidification Station inside the chamber allows for a stable delivery of humidified dilution air.
VITROCELL® 24/48
Integrated Flow Controllers
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Flow controllers integrated in climatic chamber.
VITROCELL® 24/48
Controlled Heating System
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Climatic Chamber can be heated to 37 °C to avoid condensation and enable stable humidity level.
VITROCELL® 24/48
PLUS Version
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The 8 dosimetry positions can be equipped with either Quartz Crystal Microbalances, TEM Grid Holders or stainless Dosimetry Inserts for aerosol trapping in liquids.
VITROCELL® 48/48 Plus Exposure System
for 35 mm Petri dishes
VITROCELL® 48/48
Plus Exposure Module
The VITROCELL® AMES 48 module system has been specifically designed and engineered to facilitate the research of bacteria in direct exposure to airborne substances such as gases, complex mixtures, nanoparticles and fibers.
Only 1 system is required: 7 dilutions with 6 petri dishes for exposure to the substances and 6 Petri dishes in the same system for clean air control.
A complete dose / response profile can be obtained in one experiment. The bacteria are directly exposed using controlled flow rates of the aerosol.
After exposure, the cultures are further processed to measure the number of revertants.
VITROCELL® 48/48
Base module
The base module has been designed for 48 Petri dishes.
The base module is made of aluminum with compartments of stainless steel. It is autoclavable at 121° C (250° F) for 20 min. This module is renowned in scientific circles for its reliability and durability.
VITROCELL® 48/48
Easy access to all components
VITROCELL® 48/48
Heating System
Heating Systems for base module and climatic chamber to avoid effects of aerosol condensation.
VITROCELL® 48/48
Controlled inlet flow
VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
VITROCELL® 48/48
Exposure module with 7th row on drawer
VITROCELL® 48/48 Features
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Suitable for Petri dishes (diameter 35 mm)
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Hyperboloid geometry of inlets for optimized
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particle deposition and distribution
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Direct flow control for each inlet
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Base module compartments made of titanium for maximum durability
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Autoclavable at 121° C (250° F)
VITROCELL® 48/48
Petri dishes
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The module has been designed for 35 mm Petri dishes.
VITROCELL® 48/48
Microbalance Sensor – Additional option
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The Humidification Station inside the chamber allows for a stable delivery of humidified dilution air.
VITROCELL® 96
for 96-well HTS plates
VITROCELL® 96
The VITROCELL® 96 Exposure System has been specifically designed and engineered to facilitate the research of human cell cultures in direct exposure to airborne substances such as gases, complex mixtures and particles including nanoparticles. The system authentically simulates the conditions of human physiological exposure. Up to 11 dilutions with 8 replicates are used for for exposure to test substances and the 12th insert row for clean air control with 8 replicates.
Each dilution represents a different dose concentration, so that a complete dose/response profile can be obtained in one experiment.
VITROCELL® 96 Climatic Chamber
The system is integrated in the Climatic Chamber which enables a stable temperature of 37° C for all components. The benefits are condensation-free operation and a stable humidity level for exposure. The integrated humidification system features a pre-heating of dilution air. All flow controllers are mounted inside the chamber for constant temperature and increased humidity output.
Corning HTS Transwell 96-well Plate
The cell cultures are exposed at the Air/Liquid Interface on 96 membranes of the HTS plate using low flow rates of the aerosol.
After exposure, the cells are further processed to measure a wide range of endpoints e.g. cytotoxicity, genotoxicity, proliferation, cellular and oxidative stress as well as inflammation (see also VITROCELL® assay guide).
VITROCELL® 96 Base Module
Exposure of 12 rows @ 8 replicates
The base module with 96 wells is made of electropolished stainless steel. It may be easily removed from the integrated heating base. Temperature can be programmed individually.
VITROCELL® 96 Aero exposure top
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96 inlets with indidual vacuum flow
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Special sealing and connection mechanisms guarantee a hermetic connection of the base module with the aerosol exposure top. The aerosol exposure top guides the test atmosphere at an individually controlled flow rate to each cell culture membrane.
Controlled inlet flow
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VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
Integrated Dilution Systems
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New support and quick-mounting mechanism of dilution systems and exposure top for easier handling.
Integrated Humidification Station
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The Humidification Station inside the chamber allows for a stable delivery of humidified dilution air
1. Docking Station for Base Module and Exposure Top
The docking station guarantees a hermetic connection of the base module with the aerosol exposure top. The flow rate for each of the 96 inlets is controlled by critical orifices which are connected to a vacuum pump.
2. Quick-lock Dilution Systems
The integrated 12-fold dilution system can be easily separated from the exposure head for cleaning and maitenance.
Performance Qualification
The system was evaluated in a comprehensive performance qualification using combustion cigarette aerols. As a conclusion, the system is fit for purpose.
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11 Dilution Steps: 0,5–8 l/min Air
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Cigarettes: 1R6F
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Smoking Regime: Health Canada
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Puff number: 5 Cigarettes à 8 puffs
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Puff volume: 55 ml
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Puff duration: 2s
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Exhaust duration: 8s
Continuous Flow:
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Assay: Autofluorescence
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Trapping Liquid: DMSO
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Trapping Volume: 96-well 60 μL
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Vitrocell SOP: QMD-70- 39
Features
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Base module for 96-well HTS plates
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12-fold Dilution/Distribution System
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12 mass flow controllers for humidified air
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11 dilutions @ 8 replicates
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1 clean air control @ 8 replicates
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Electronic heating system for base module and exposure head
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Exposure head with 96 critical orifices and filter system
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Climatic Chamber for condensation-free operation if using liquid aerosols
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Housing with double-doors
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Heating systems with two RH/T sensors for temperature monitoring
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Software for read-out of sensor data to controller
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Integrated humidification system